Phenotypic & functional profiling of immune cells

Investigating the phenotypic and functional profiling of immune cells represents a fundamental endeavour in immunological research, offering valuable insights into the intricate orchestration of immune responses. This multifaceted approach involves the systematic characterisation of immune cell populations based on their surface markers and effector functions, providing a nuanced understanding of their roles in health and disease.

Here in the Gavins lab, we are using cutting-edge techniques such as flow cytometry, mass cytometry (CyTOF), high-content, multiplexed image-based assays and single-cell RNA sequencing, to meticulously delineate the diverse repertoire of immune cell subsets within complex biological systems. By interrogating the expression patterns of key molecules such as cytokines, chemokines, and surface receptors, we are trying to discern the phenotypic signatures associated with distinct immune cell populations and their activation states.

We believe that by integrating phenotypic and functional data, we can unravel the dynamic interplay between different immune cell subsets and decipher the underlying mechanisms governing immune responses in health and disease contexts. Such comprehensive profiling will not only enhance our understanding of basic immunological principles but also holds immense translational potential, guiding the development of novel immunotherapies, vaccines, and diagnostic strategies. Ultimately, elucidating the phenotypic and functional landscapes of immune cells promises to illuminate new avenues for precision medicine and personalised immunomodulatory interventions.