Developing non-mammalian models for thrombosis research
Thrombosis, the pathological formation of blood clots within blood vessels, remains a major challenge in cardiovascular diseases, including stroke. Traditional mammalian models have advanced our understanding of thrombotic processes but are limited by high costs, ethical concerns, and genetic uniformity. These challenges have driven interest in non-mammalian models, such as zebrafish (Danio rerio), which offer unique advantages like optical transparency and genetic tractability. Zebrafish provide a powerful platform to study the interplay between immune cells and thrombotic mechanisms, offering valuable insights into immune response dynamics and disease progression.
This project focuses on the phenotypic and functional profiling of immune cells, a cornerstone of immunological research. By systematically characterizing immune cell populations based on surface markers and effector functions, we aim to deepen our understanding of their roles in health and disease. Advanced techniques, including single-cell RNA sequencing, flow cytometry, spatial transcriptomics and 3D/4D behavioural profiling, will enable unprecedented resolution in profiling immune cell diversity and functionality. These cutting-edge methodologies will facilitate the discovery of novel biomarkers and therapeutic targets, driving innovation in immune and thrombotic research.
This work is funded by: CHMLS Dean's Studentship
